Background: Current data suggest that physiologic doses of vitamin B-6 have no significant homocysteine-lowering effect.
It is possible that an effect of vitamin B-6 was missed in previous trials because of a much greater effect of folic acid, vitamin
B-12, or both. Objective: The aim of this study was to investigate the effect of low-dose vitamin B-6 supplementation on fasting total homocysteine (tHcy) concentrations in healthy elderly persons who were made replete with folate and riboflavin.

Design: Twenty-two healthy elderly persons aged 63–80 y were supplemented with a low dose of vitamin B-6 (1.6 mg/d) for 12 wk
in a randomized, double-blind, placebo-controlled trial after repletion with folic acid (400 g/d for 6 wk) and riboflavin (1.6 mg/d
for 18 wk); none of the subjects had a vitamin B-12 deficiency.

Results: Folic acid supplementation lowered fasting tHcy by 19.6% (P < 0.001). After folic acid supplementation, baseline tHcy concentrations ranged from 6.22 to 23.52 mol/L and 10 subjects had suboptimal vitamin B-6 status (plasma pyridoxal- P < 20 nmol/L). Two-way analysis of variance showed that the significant improvement in vitamin B-6 status in response to vitamin B-6 supplementation (on the basis of both pyridoxal-P and the erythrocyte aspartate aminotransferase activation coefficient) was reflected in a significant reduction in plasma tHcy of 7.5%.

Conclusions: Low-dose vitamin B-6 effectively lowers fasting plasma tHcy in healthy subjects who are both folate and riboflavin
replete. This suggests that any program aimed at the treatment or prevention of hyperhomocysteinemia should include vitamin B-6
supplementation. Am J Clin Nutr 2001;73:759–64.